Inhibition of Aspergillus niger phosphate solubilization by fluoride released from rock 1 phosphate 2 Running title : Inhibition of fungal P solubilization by fluoride 3 4

Inhibition of Aspergillus niger phosphate solubilization by fluoride released from rock 1 phosphate 2 Running title: Inhibition of fungal P solubilization by fluoride 3 4 Gilberto de Oliveira Mendes, Nikolay Bojkov Vassilev, Victor Hugo Araújo Bonduki, 5 Ivo Ribeiro da Silva, José Ivo Ribeiro Júnior, Maurício Dutra Costa 6 7 a Department of Microbiology, Federal University of Viçosa, Brazil 8 b Department of Chemical Engineering, Faculty of Sciences, University of Granada, Spain 9 c Department of Soil Science, Federal University of Viçosa, Brazil 10 d Department of Statistics, Federal University of Viçosa, Brazil 11 e Researcher of the National Council for Scientific and Technological Development 12 (CNPq), Brazil 13 14 Abstract 15 The simultaneous release of various chemical elements with inhibitory potential for 16 phosphate solubilization from rock phosphate (RP) was studied in this work. Al, B, Ba, Ca, 17 F, Fe, Mn, Mo, Na, Ni, Pb, Rb, Si, Sr, V, Zn, and Zr were released concomitantly with P 18 during the solubilization of Araxá RP (Brazil), but only F showed inhibitory effects on the 19 process at the concentrations detected in the growth medium. Besides P solubilization, 20 # Corresponding author: Laboratório de Associações Micorrízicas, Instituto de Biotecnologia Aplicada à Agropecuária (BIOAGRO), Av. P. H. Rolfs, s/n, Campus, Viçosa, MG, 36570-000, Brazil. Email: [email protected]. Telephone: +55 31 38992965 Copyright © 2013, American Society for Microbiology. All Rights Reserved. Appl. Environ. Microbiol. doi:10.1128/AEM.01487-13 AEM Accepts, published online ahead of print on 14 June 2013